A Radioimmunoassay for Rat Serum Parathyroid Hormone using N-terminal of PTH
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چکیده
منابع مشابه
A radioimmunoassay for rat serum parathyroid hormone using N-terminal of PTH.
Partially purified bovine parathyroid hormone (PTH) produced two kinds of antisera, GP-100 reacting with 125I-labeled bovine PTH (b-PTH 1-84) alone and GP-M reacting with 125I-labeled synthetic N-terminal portion of bovine PTH (b-PTH 1-34) as well as b-PTH 1-84. Rat serum PTH reacted with the latter but not in the former, suggesting a similarity to b-PTH 1-34. Rat serum PTH became undetectabl...
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A new radioimmunoassay for human parathyroid hormone (PTH) in serum, which can measure the hormone present in 94% of the normal sera tested, is described. It is based on the ability of human PTH to compete with (131)I-labeled bovine PTH for binding to an antiserum directed against porcine PTH. This antiserum distinguishes between human PTH extracted from parathyroid adenomata and that present i...
متن کاملConditions for a radioimmunoassay of serum parathyroid hormone.
A radioimmunoassay for parathyroid hormone is described that circumvents some previous difficulties. Parathyroid hormone binds strongly to other serum components. Consequently, a “control” curve is set up for each specimen, the specimen consisting of serum, diluent, and radiolabel, but no antibody. Final calculations are then based on the difference between the control curve and the curve obtai...
متن کاملRadioimmunoassay specific for amino (N) and carboxyl (C) terminal portion of parathyroid hormone.
A radioimmunoassay specific for the amino (N) terminal portion of the parathyroid hormone (PTH) molecule (N-PTH radioimmunoassay) has been developed by iodinating synthetic 1-34bovine PTH (1-34bPTH) and using commercially available bPTH antiserum. A radioimmunoassay specific for the carboxyl (C) terminal (C-PTH radioimmunoassay) has been carried out by adding enough amount of 1-34bPTH to the PT...
متن کاملC-terminal parathyrin (parathyroid hormone) radioimmunoassay in serum with commercially available reagents.
We describe a sequential double-antibody assay for measuring C-terminal parathyrin in serum with commercially available reagents. Intact bovine hormone, used as a working standard, is iodinated by conventional Chloramine T procedures. The antibody affinity is characteristic of high affinity binding (1.4 to 1.6 x 10(10) L/mol of intact parathyrin). The antibody also cross reacts with a C-termina...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1975
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.22.159